Detection of bovine herpesvirus type 1 and bovine ephemeral fever virus antigens in cattle lymph nodes using three immunological assays

Isolation of Bovine Ephemeral Fever Virus in Iran

Isolation of Bovine Herpesvirus-1 (BoHV-1) From Latently Infected/Carrier Cattle in Ahvaz

Bovine herpesvirus 1 (BoHV-1) is one of the most important infectious viruses of cattle which is considered as the causative agent of several clinical conditions in this species. Hence, we tried to isolate a local strain of the virus from carrier cattle in Ahvaz based on a strategy for reactivation of the latent infection in hosts having antibodies against the virus. Thus, 20 cattle were examin...

Production of Monoclonal Antibody against Prokaryotically Expressed G1 Protein of Bovine Ephemeral Fever Virus

Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...

Evolution of bovine ephemeral fever virus in the Australian episystem.

Bovine ephemeral fever virus (BEFV) is an arthropod-borne rhabdovirus that causes a debilitating disease of cattle in Africa, Asia, and Australia; however, its global geodynamics are poorly understood. An evolutionary analysis of G gene (envelope glycoprotein) ectodomain sequences of 97 BEFV isolates collected from Australia during 1956 to 2012 revealed that all have a single common ancestor an...

Serological and genomic detection of bovine leukemia virus in human and cattle samples

Bovine leukemia virus (BLV) is a retrovirus responsible for lymphoproliferative disorders in cattle. Although infections of BLV in animals are well known, little is known about its capacity to infect humans. This study investigated the presence of anti-BLV antibodies and BLV proviruses in human and cattle samples. An indirect enzyme-linked immunosorbent assay (ELISA) was used to detect anti-BL...